SARS-CoV Spike (SARS-CoV S)
(SARS-Coronavirus Spike Protein (SARS-CoV S))
Type de proteíne
Recombinant
Attributs du protein
RBD
Origine
SARS Coronavirus (SARS-CoV)
Source
HEK-293 Cells
Purification/Conjugué
Cette SARS-CoV Spike protéine est marqué à la His-Avi Tag.
Séquence
Arg306-Phe527
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized SARS Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. See testing image for detail.
SARS-CoV S
Origine: SARS Coronavirus (SARS-CoV)
Hôte: Escherichia coli (E. coli)
Recombinant
>95 %
ELISA, WB
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
SARS-CoV Spike (SARS-CoV S)
(SARS-Coronavirus Spike Protein (SARS-CoV S))
S protein RBD, S glycoprotein RBD, Spike protein RBD,The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Poids moléculaire
27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.