> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-SEMA3A Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 0.9μg/ml determined by ELISA. See testing image for detail.
SEMA3A
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
Stock
-80 °C
Stockage commentaire
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Semaphorin-3A, Semaphorin III, Sema III, Semaphorin-D, Sema D, coll-1, collapsin 1, Hsema-I, Hsema-III, MGC133243, SEMA1, Sema3A, SEMAD, SEMAIII, SEMAL, semaphorin L,The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.