Cette SIGLEC15 protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Cynomolgus Siglec-15/CD33L3 Protein
Séquence
Met60-Thr322
Attributs du produit
Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-Human Siglec-15 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus Siglec-15, His Tag with the EC50 of 23.2ng/ml determined by ELISA. See testing image for detail.
SIGLEC15
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM PB, 500 mM NaCl, 0.1M L-arginine ( pH 6.0).
Stock
-80 °C
Stockage commentaire
Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Poids moléculaire
29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Tris-Bis PAGE result.