Recombinant Cynomolgus SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus SPARC, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. See testing image for detail.
SPARC
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
SPARC
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein
Sujet
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Poids moléculaire
33.82 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.