TGFB2 Protein (AA 303-414)

N° du produit ABIN7275710

Protéine Recombinant TGFB2 exprimée dans HEK-293 Cells.

Aperçu rapide pour TGFB2 Protein (AA 303-414) (ABIN7275710)

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE

Détail du produit

Attributs du protein: AA 303-414

Fonction: Human Mature TGF beta 2 Protein

Séquence: Ala303-Ser414

Attributs du produit: Recombinant Human Mature TGF beta 2 Protein is expressed from HEK293 without tag. It contains Ala303-Ser414.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Mature TGF beta 2, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test).,Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 0.1 - 1 ng/ml (QC Test).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Autre désignation: TGF beta 2

Sujet: Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.

Poids moléculaire: 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

UniProt: P61812

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus