TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Type de proteíne
Recombinant
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF1B protéine est marqué à la mFc Tag.
Séquence
Leu23-Asp257
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNFR2 at 5μg/ml (100μl/Well). Dose response curve for Human TNF alpha, His Tag with the EC50 of 0.17μg/ml determined by ELISA. See testing image for detail.
TNFRSF1B
Origine: Souris
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
CD120b, Etanercept, p75TBPII, p75TNFR, TNF RII, TNF-R2, TNF-R75, TNFR80, TNFRSF1B, TNFR2, TBPII, TNFBR, TNFR1B,Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.
Poids moléculaire
51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.