TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
Type de proteíne
Recombinant
Attributs du protein
AA 34-239
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF10A protéine est marqué à la Fc Tag.
Fonction
Human TRAIL R1/DR4/TNFRSF10A Protein
Séquence
Pro34-Asn239
Attributs du produit
Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro34-Asn239.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA. See testing image for detail.
TNFRSF10A
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
SDS, WB, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
APO2 Protein, CD261 Protein, DR4 Protein, TRAILR-1 Protein, TRAILR1 Protein, TNFRSF10A Protein, TNF receptor superfamily member 10a Protein, tumor necrosis factor receptor superfamily member 26 Protein, tumor necrosis factor receptor superfamily, member 10a Protein, TNFRSF10A Protein, LOC510798 Protein
Sujet
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis selectively via its interaction with the death receptors TRAILR1/DR4 and TRAILR2/DR5 in a wide range of cancers, while sparing normal cells. Despite its tremendous potential for cancer therapeutics, the translation of TRAIL into the clinic has been confounded by TRAIL-resistant cancer populations.
Poids moléculaire
48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.