TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
Type de proteíne
Recombinant
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF10B protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Ile56-Glu182
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-TRAIL R2 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R2, His Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.
TNFRSF10B
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
CD262, DR5, DR5TRICK2B, Fas-like protein, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICKB, ZTNFR9, KILLER,DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Poids moléculaire
17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.