Recombinant Human TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly25-Lys231.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TSLPR, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.
CRLF2
Origine: Humain
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CRLF2
Origine: Souris
Hôte: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
TSLP is an epithelial cell-derived cytokine synthesized in response to various stimuli, including protease allergens and microorganisms like viruses and bacteria. Biological functions of TSLP require heterodimer formation between the TSLP receptor (TSLPR) and IL-7 receptor-α, which polarize dendritic cells to induce type 2 inflammation and directly expand and/or activate Th2 cells, group 2 innate lymphoid cells, basophils, and other immune cells.
Poids moléculaire
25.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.