TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
Type de proteíne
Recombinant
Attributs du protein
AA 72-251
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFSF15 protéine est marqué à la His tag.
Fonction
Human TNFSF15 Protein
Séquence
Leu72-Leu251
Attributs du produit
Recombinant Human TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu72-Leu251.
Pureté
> 90 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNFSF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA. See testing image for detail.
TNFSF15
Origine: Souris
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNFSF15
Origine: Cynomolgus
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 20 mM PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFSF15
(Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))
TTPAL Protein, TL1 Protein, TL1A Protein, VEGI Protein, VEGI192A Protein, Tl1 Protein, Tl1a Protein, Vegi Protein, tumor necrosis factor superfamily member 15 Protein, TNF superfamily member 15 Protein, tumor necrosis factor (ligand) superfamily, member 15 Protein, TNFSF15 Protein, Tnfsf15 Protein
Sujet
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Poids moléculaire
21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.