HLA-E*01:03 (Tetramer) protein (His-Avi Tag)

N° du produit ABIN7454271

Détail du produit

Antigène: HLA-E*01:03

Type de proteíne: Recombinant

Attributs du protein: Tetramer

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His-Avi Tag

Séquence: Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide

Attributs du produit: Recombinant Human HLA-E*01:03 Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: HLA-E*01:03

Sujet: HLAE, sHLA-E, HLAE, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, MHC, QA1. HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with ≤2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.

Poids moléculaire: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.