HLA-E*01:03 (AA 22-302), (Tetramer) (Active) protein (His-Avi Tag,Peptide (RIIPRHLQL))

N° du produit ABIN7454271

Protéine Recombinant HLA-E*01:03 exprimée dans HEK-293 Cells.

Aperçu rapide pour HLA-E*01:03 (AA 22-302), (Tetramer) (Active) protein (His-Avi Tag,Peptide (RIIPRHLQL)) (ABIN7454271)

Antigène: HLA-E*01:03

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 22-302, Tetramer

Purification/Conjugué: His-Avi Tag,Peptide (RIIPRHLQL)

Fonction: Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein

Séquence: Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide

Attributs du produit: Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200).,Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).,Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).,Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.,The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-E*01:03

Sujet: HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.

Poids moléculaire: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: P13747