HLA-A*03:01 (AA 25-305), (G12V), (Tetramer) (Active) protein (His-Avi Tag,KRAS G12V)

N° du produit ABIN7454293

Cette protéine Recombinant HLA-A*03:01 est produite dans HEK-293 Cells.

Aperçu rapide pour HLA-A*03:01 (AA 25-305), (G12V), (Tetramer) (Active) protein (His-Avi Tag,KRAS G12V) (ABIN7454293)

Antigène: HLA-A*03:01

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 25-305, G12V, Tetramer

Purification/Conjugué: His-Avi Tag,KRAS G12V

Fonction: Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein

Séquence: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide

Specificité: Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAVGVGK

Attributs du produit: Recombinant Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody captured on CM5 Chip via Protein A can bind Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200).,Immobilized Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test).,Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.,The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-A*03:01

Sujet: Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

Poids moléculaire: 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

NCBI Accession: NP_002107