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HLA-A*03:01 (Tetramer) protein (KRAS G12V)

Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
N° du produit ABIN7454293
  • Antigène
    HLA-A*03:01
    Type de proteíne
    Recombinant
    Attributs du protein
    Tetramer
    Origine
    Humain
    Source
    • 10
    • 1
    HEK-293 Cells
    Purification/Conjugué
    KRAS G12V
    Fonction
    Human HLA-A*03:01 & B2M & KRAS G12V (VVVGAVGVGK) Tetramer Protein
    Séquence
    Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and VVVGAVGVGK peptide
    Specificité
    Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAVGVGK
    Attributs du produit
    Recombinant Human HLA-A*03:01 & B2M & KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.
    Pureté
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA (QC Test). The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    HLA-A*03:01
    Sujet
    Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
    Poids moléculaire
    258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
    NCBI Accession
    NP_002107
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