HLA-A*02:01 (Monomer) protein (WT-1)

N° du produit ABIN7454364

Détail du produit

Antigène: HLA-A*02:01

Type de proteíne: Recombinant

Attributs du protein: Monomer

Origine: Humain, Souris

Source: HEK-293 Cells

Purification/Conjugué: WT-1

Fonction: Chimeric HLA-A*02:01 (mα3) & B2M & WT-1 (RMFPNAPYL) Monomer Protein

Séquence: Gly25-Thr206 (Human HLA-A*02:01 α1&α2) & Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide

Specificité: Uni-Prot: A0A140T913 (Human HLA-A*02:01 α1, α2), P01897 (Mouse H-2Ld α3), P61769 (B2M), RMFPNAPYL

Attributs du produit: Recombinant Chimeric HLA-A*02:01(mα3) & B2M & WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A*02:01 α1 & α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.1ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-A*02:01

Sujet: The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

Poids moléculaire: 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.

UniProt: A0A140T913