BDNF Protein (AA 129-247)

N° du produit ABIN7504243

Cette protéine Recombinant BDNF est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour BDNF Protein (AA 129-247) (ABIN7504243)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Rat, Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE

Détail du produit

Attributs du protein: AA 129-247

Fonction: Human/Mouse/Rat BDNF Protein

Séquence: His129-Arg247(Human) / His131-Arg249(Mouse/Rat)

Attributs du produit: Recombinant Human/Mouse/Rat BDNF Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.01 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test).,Human/Mouse/Rat BDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF

Sujet: The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.

Poids moléculaire: 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity