CD300a Protein (CD300A) (AA 18-180) (AVI tag,Biotin)

N° du produit ABIN7504260

Cette protéine Recombinant CD300a est produite dans HEK-293 Cells.

Aperçu rapide pour CD300a Protein (CD300A) (AA 18-180) (AVI tag,Biotin) (ABIN7504260)

Antigène: CD300a (CD300A)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 18-180

Purification/Conjugué: Cette CD300a protéine est marqué à la AVI tag,Biotin.

Fonction: Biotinylated Human CD300A Protein

Séquence: Leu18-Pro180

Attributs du produit: Recombinant Biotinylated Human CD300A Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Leu18-Pro180.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD300A, Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA (QC Test).,Biotinylated Human CD300A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD300A is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD300a (CD300A)

Autre désignation: CD300A

Sujet: CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI), however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.

Poids moléculaire: 19.46 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process