CEACAM5 Protein (AA 35-685) (Biotin,His-Avi Tag)

N° du produit ABIN7504291

Cette protéine Recombinant CEACAM5 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CEACAM5 Protein (AA 35-685) (Biotin,His-Avi Tag) (ABIN7504291)

Antigène: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 35-685

Purification/Conjugué: Cette CEACAM5 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Human CEACAM-5/CD66e Protein

Séquence: Lys35-Ala685

Attributs du produit: Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Lys35-Ala685.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA (QC Test).,Biotinylated Human CEACAM-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CEACAM-5 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Autre désignation: CEACAM-5

Sujet: Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

Poids moléculaire: 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on Bis-Tris PAGE result.

UniProt: P06731