FLT3 Protein (AA 27-541) (Biotin,His-Avi Tag)

N° du produit ABIN7504335

Cette protéine Recombinant FLT3 est produite dans CHO Cells.

Aperçu rapide pour FLT3 Protein (AA 27-541) (Biotin,His-Avi Tag) (ABIN7504335)

Antigène: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 27-541

Purification/Conjugué: Cette FLT3 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Human FLT3/Flk-2 Protein

Séquence: Asn27-Asn541

Attributs du produit: Recombinant Biotinylated Human FLT3/Flk-2 Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Asn27-Asn541.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human FLT3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).,Biotinylated Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human FLT3 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Autre désignation: FLT3

Sujet: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Poids moléculaire: 61.20 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

UniProt: P36888

Pathways: Signalisation RTK