HLA-A*02:01 (AA 25-305), (Monomer) (Active) protein (Biotin,PRAME,His-Avi Tag)

N° du produit ABIN7504364

Protéine Recombinant HLA-A*02:01 exprimée dans HEK-293 Cells.

Aperçu rapide pour HLA-A*02:01 (AA 25-305), (Monomer) (Active) protein (Biotin,PRAME,His-Avi Tag) (ABIN7504364)

Antigène: HLA-A*02:01

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 25-305, Monomer

Purification/Conjugué: Biotin,PRAME,His-Avi Tag

Fonction: Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Séquence: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and SLLQHLIGL peptide

Specificité: Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M), SLLQHLIGL

Attributs du produit: Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).,Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer (Cat. No.MHC-HM443B) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.,The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-A*02:01

Sujet: PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Poids moléculaire: 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result.

UniProt: A0A140T913