HLA-A*03:01 (AA 25-305), (Tetramer) (Active) protein (His-Avi Tag,PE,no Peptide)
Aperçu rapide pour HLA-A*03:01 (AA 25-305), (Tetramer) (Active) protein (His-Avi Tag,PE,no Peptide) (ABIN7504389)
Antigène
Type de proteíne
Activité biologique
Origine
Source
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Attributs du protein
- AA 25-305, Tetramer
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Purification/Conjugué
- His-Avi Tag,PE,no Peptide
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Fonction
- PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein
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Séquence
- Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)
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Specificité
- Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M)
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Attributs du produit
- Recombinant PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin.It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).
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Stérilité
- 0.22 μm filtered
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Biological Activity Comment
- Immobilized PE-Labeled Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
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Indications d'application
- Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
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Commentaires
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Peptide Ready: This HLA protein ist absent from peptide, namely peptide receptive MHC. It can be loaded with antigenic peptides matching its type.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22 μm filtered solution in PBS, 300 mM NaCl ( pH 7.4).
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Stock
- -80 °C
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Stockage commentaire
- Valid for 6 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 6 months
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- HLA-A*03:01
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Sujet
- Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
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NCBI Accession
- NP_002107
Antigène
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