HLA-A*24:02 (AA 25-305), (Monomer) (Active) protein (His-Avi Tag,no Peptide)

N° du produit ABIN7504413

Cette protéine Recombinant HLA-A*24:02 est produite dans HEK-293 Cells.

Aperçu rapide pour HLA-A*24:02 (AA 25-305), (Monomer) (Active) protein (His-Avi Tag,no Peptide) (ABIN7504413)

Antigène: HLA-A*24:02

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 25-305, Monomer

Purification/Conjugué: His-Avi Tag,no Peptide

Fonction: Human Peptide Ready HLA-A*24:02&B2M Monomer Protein

Séquence: Gly25-Thr305(HLA-A*24:02) & Ile21-Met119(B2M)

Specificité: Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M)

Attributs du produit: Recombinant Human Peptide Ready HLA-A*24:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02) and Ile21-Met119(B2M).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Peptide Ready HLA-A*24:02&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).,Human Peptide Ready HLA-A*24:02&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Peptide Ready HLA-A*24:02&B2M Monomer is greater than 95% as determined by SEC-HPLC.

Information d'application

Indications d'application: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Commentaires:

Peptide Ready: This HLA protein ist absent from peptide, namely peptide receptive MHC. It can be loaded with antigenic peptides matching its type.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-A*24:02

Sujet: Peptide Ready HLA-A*24:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*24:02. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Poids moléculaire: 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.