HLA-E*01:03 (AA 22-302), (Monomer) (Active) protein (His-Avi Tag,no Peptide)

N° du produit ABIN7504417

Cette protéine Recombinant HLA-E*01:03 est exprimée dans HEK-293 Cells.

Aperçu rapide pour HLA-E*01:03 (AA 22-302), (Monomer) (Active) protein (His-Avi Tag,no Peptide) (ABIN7504417)

Antigène: HLA-E*01:03

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 22-302, Monomer

Purification/Conjugué: His-Avi Tag,no Peptide

Fonction: Human Peptide Ready HLA-E*01:03&B2M Monomer Protein

Séquence: Gly22-Thr302(HLA-E*01:03) & Ile21-Met119(B2M)

Specificité: Uni-Prot: P13747 (HLA-E*01:03), P61769 (B2M)

Attributs du produit: Recombinant Human Peptide Ready HLA-E*01:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03) and Ile21-Met119(B2M).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Peptide Ready HLA-E*01:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test).,Human Peptide Ready HLA-E*01:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Peptide Ready HLA-E*01:03&B2M Monomer-is greater than 95% as determined by SEC-HPLC.

Information d'application

Indications d'application: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Commentaires:

Peptide Ready: This HLA protein ist absent from peptide, namely peptide receptive MHC. It can be loaded with antigenic peptides matching its type.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HLA-E*01:03

Sujet: HLA-E*01:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Poids moléculaire: 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: P13747