IL-10RA Protein (AA 22-233) (Biotin,His tag)

N° du produit ABIN7504430

Protéine Recombinant IL-10RA exprimée dans HEK-293 Cells.

Aperçu rapide pour IL-10RA Protein (AA 22-233) (Biotin,His tag) (ABIN7504430)

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Cynomolgus

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 22-233

Purification/Conjugué: Cette IL-10RA protéine est marqué à la Biotin,His tag.

Fonction: Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)

Séquence: His22-Asn233

Attributs du produit: Recombinant Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains His22-Asn233.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA.,Biotinylated Cynomolgus IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Autre désignation: IL-10 R alpha

Sujet: The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

Poids moléculaire: 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5WJ66

Pathways: Growth Factor Binding