SEZ6 Protein (AA 20-925) (Biotin,His-Avi Tag)

N° du produit ABIN7504525

Cette protéine Recombinant SEZ6 est exprimée dans HEK-293 Cells.

Aperçu rapide pour SEZ6 Protein (AA 20-925) (Biotin,His-Avi Tag) (ABIN7504525)

Antigène: SEZ6 (Seizure Related 6 Homolog (SEZ6))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 20-925

Purification/Conjugué: Cette SEZ6 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Human SEZ6 Protein

Séquence: Leu20-His925

Attributs du produit: Recombinant Biotinylated Human SEZ6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-His925.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).,Biotinylated Human SEZ6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-SEZ6 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human SEZ6, His-Avi Tag with an affinity constant of 3.31 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SEZ6 (Seizure Related 6 Homolog (SEZ6))

Autre désignation: SEZ6

Sujet: Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.

Poids moléculaire: 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Bis-Tris PAGE result.

UniProt: Q53EL9

Pathways: Synaptic Membrane