SKP1 Protein (AA 2-163) (AVI tag,Biotin,His tag)

N° du produit ABIN7504529

Protéine Recombinant SKP1 exprimée dans HEK-293 Cells.

Aperçu rapide pour SKP1 Protein (AA 2-163) (AVI tag,Biotin,His tag) (ABIN7504529)

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 2-163

Purification/Conjugué: Cette SKP1 protéine est marqué à la AVI tag,Biotin,His tag.

Fonction: Biotinylated Human SKP1 Protein

Séquence: Pro2-Lys163

Attributs du produit: Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human SKP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Autre désignation: SKP1

Sujet: The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.

Poids moléculaire: 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.

UniProt: P63208

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases