Recombinant Mouse tPA Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559).
PLAT
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
Poids moléculaire
85.00 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 35-40 kDa (Chain A) and 30-35 kDa (Chain B) based on Tris-Bis PAGE result.