p53 Protein (AA 1-386) (His tag)

N° du produit ABIN7504911

Détail du produit

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Type de proteíne: Recombinant

Attributs du protein: AA 1-386

Origine: Boeuf (Vache)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette p53 protéine est marqué à la His tag.

Séquence: Met1-Asp386

Attributs du produit: A DNA sequence encoding the Bovine P53 protein (P67939-1) (Met1-Asp386) was expressed with a N-HIS.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: P53 (TP53 Produits)

Synonymes: BCC7 Protein, LFS1 Protein, P53 Protein, TRP53 Protein, p53 Protein, Trp53 Protein, brp53 Protein, drp53 Protein, etID22686.5 Protein, fb40d06 Protein, wu:fb40d06 Protein, zgc:111919 Protein, Tp53 Protein, bbl Protein, bfy Protein, bhy Protein, p44 Protein, tp53 Protein, Xp53 Protein, TP53 Protein, tumor protein p53 Protein, cellular tumor antigen p53 Protein, transformation related protein 53 Protein, tumor protein p53 L homeolog Protein, TP53 Protein, CpipJ_CPIJ002758 Protein, Tp53 Protein, tp53 Protein, Trp53 Protein, tp53.L Protein

Sujet:

Abbreviation: P53,TP53

Target Synonym: Cellular tumor antigen p53,TP53,Tumor suppressor p53,

Background: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2.

Poids moléculaire:

Calculated MW: 42.35 kDa

Observed MW: 55 kDa

UniProt: P67939-1

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg