RAC1 Protein (AA 1-189) (His tag)

N° du produit ABIN7505002

Détail du produit

Antigène: RAC1

Type de proteíne: Recombinant

Attributs du protein: AA 1-189

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette RAC1 protéine est marqué à la His tag.

Séquence: Met1-Cys189

Attributs du produit: A DNA sequence encoding theHuman Rac1 protein (P63000) (Met1-Cys189) was expressed with a N-His.

Pureté: > 90 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: RAC1

Abstract: RAC1 Produits

Synonymes: Rac-1 Protein, TC-25 Protein, p21-Rac1 Protein, AL023026 Protein, D5Ertd559e Protein, RAC2 Protein, CRAC1A Protein, rac1 Protein, wu:fd16e02 Protein, zgc:55823 Protein, zgc:55917 Protein, zgc:86934 Protein, 2248 Protein, CG2248 Protein, D-Rac Protein, D-Rac 1 Protein, D-Rac1 Protein, DRac Protein, DRac1 Protein, DRacA Protein, Dm Rac1 Protein, DmRAC Protein, DmRAC1 Protein, Dmel\CG2248 Protein, Dmrac1 Protein, Drac Protein, Drac1 Protein, Drac1a Protein, DracA Protein, GTPase Rac1 Protein, P40792 Protein, RAC1 Protein, Rac Protein, Rac GTPase Protein, Rac3 Protein, RacA Protein, dRac Protein, dRac1 Protein, drac1 Protein, rac Protein, Xrac Protein, mig5 Protein, p21-rac1 Protein, tc-25 Protein, Rac family small GTPase 1 Protein, RAS-related C3 botulinum substrate 1 Protein, ras-related C3 botulinum toxin substrate 1 Protein, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Protein, Rac family small GTPase 1a Protein, CG2248 gene product from transcript CG2248-RB Protein, Rac family small GTPase 1 L homeolog Protein, ras homolog family member G Protein, RAC1 Protein, Rac1 Protein, rac1a Protein, rac1.L Protein, RHOG Protein

Sujet:

Abbreviation: Rac1

Target Synonym: Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1

Background: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.

Poids moléculaire:

Calculated MW: 21.1 kDa

Observed MW: 27 kDa

UniProt: P63000

Pathways: Signalisation WNT, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling