RAD1 Protein (AA 1-282) (His tag)
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- Antigène Voir toutes RAD1 Protéines
- RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-282
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette RAD1 protéine est marqué à la His tag.
- Séquence
- Met 1-Ser 282
- Attributs du produit
- A DNA sequence encoding the Human RAD1 protein (O60671) (Met 1-Ser 282) was expressed with a N-His&C-His tag.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product RAD1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Date de péremption
- 12 months
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- Antigène
- RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))
- Autre désignation
- RAD1 (RAD1 Produits)
- Synonymes
- HRAD1 Protein, REC1 Protein, zgc:56502 Protein, zgc:77779 Protein, RAD1 Protein, xrad1 Protein, DKFZp459H1127 Protein, RAD1 checkpoint DNA exonuclease Protein, RAD1 homolog (S. pombe) Protein, RAD1 Protein, Rad1 Protein, rad1 Protein
- Sujet
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Abbreviation: RAD1
Target Synonym: Cell cycle checkpoint protein RAD1,RAD1, DNA repair exonuclease rad1 homolog,Rad1-like DNA damage checkpoint protein
Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
- Poids moléculaire
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Calculated MW: 30.91 kDa
Observed MW: 35 kDa
- UniProt
- O60671
- Pathways
- M Phase
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