Retinoblastoma 1 Protein (RB1) (AA 229-450) (His tag)

N° du produit ABIN7505541

Détail du produit

Antigène: Retinoblastoma 1 (RB1)

Type de proteíne: Recombinant

Attributs du protein: AA 229-450

Origine: Rat

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Retinoblastoma 1 protéine est marqué à la His tag.

Séquence: Arg 229-Met 450

Attributs du produit: A DNA sequence encoding the Rat RB1 protein (P33568) (Arg 229-Met 450) was expressed with N-His tag.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: RB1 (RB1 Produits)

Synonymes: OSRC Protein, RB Protein, p105-Rb Protein, pRb Protein, pp110 Protein, Rb Protein, Rb-1 Protein, P105-RB Protein, PP110 Protein, RB11 Protein, zgc:154147 Protein, RB transcriptional corepressor 1 Protein, RB transcriptional corepressor like 1 Protein, retinoblastoma 1 Protein, RB1 Protein, RBL1 Protein, Rb1 Protein, rb1 Protein

Sujet:

Abbreviation: RB1

Target Synonym: OSRC,osteosarcoma,p105-Rb,pp110,pRb,RB,RB1,retinoblastoma 1,retinoblastoma suspectibility protein,retinoblastoma-associated protein

Background: Retinoblastoma 1 protein (RB-1, also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family. Rat RB-1 is 920 amino acids in length. The protein contains a Pocket domain (aa 366-763), which is comprised of two other domains, domain A (aa 366-572) and domain B (aa 632-763), and a ""spacer"" (aa 573-631). The Pocket domain binds to threonine-phosphorylated domain C (aa 763-920), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes. RB-1 is expressed in the retina. The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB-1 lead to the childhood cancer retinoblastoma.

Poids moléculaire:

Calculated MW: 24.31 kDa

Observed MW: 32 kDa

UniProt: P33568

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy