MICB Protein (AA 1-298) (His tag)
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- Antigène Voir toutes MICB Protéines
- MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-298
- Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette MICB protéine est marqué à la His tag.
- Application
- ELISA
- Séquence
- Met 1-Gly 298
- Attributs du produit
- A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.
- Pureté
- > 98 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- <1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Immobilized human MICB-His at 10 ug/ml (100 ul/well) can bind human NKG2D, The EC50 of human NKG2D is 0.52-1. 2 ug/ml.
- Top Product
- Discover our top product MICB Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Date de péremption
- 12 months
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- Antigène
- MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
- Autre désignation
- MICB (MICB Produits)
- Synonymes
- PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein
- Sujet
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Abbreviation: MICB
Target Synonym: PERB11.2
Background: MHC class I polypeptide-related sequence B; also known as MICB; is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA; a closely related protein; both of which contain three extracellular immunoglobulin-like domains; but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen; binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells; CD8+αβ T cells; and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells; but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance; viral infections; and autoimmune diseases.
- Poids moléculaire
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Calculated MW: 33 kDa
Observed MW: 45-50 kDa
- UniProt
- Q29980
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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