Myeloperoxidase Protein (MPO) (AA 49-745) (His tag)
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- Antigène Voir toutes Myeloperoxidase (MPO) Protéines
- Myeloperoxidase (MPO)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 49-745
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette Myeloperoxidase protéine est marqué à la His tag.
- Séquence
- Ala49-Ser745
- Attributs du produit
- Recombinant Human Myeloperoxidase is produced by our Mammalian expression system and the target gene encoding Ala49-Ser745 is expressed with a 10His tag at the C-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- <1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product MPO Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from a 0.2 um filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Date de péremption
- 12 months
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- Antigène
- Myeloperoxidase (MPO)
- Autre désignation
- Myeloperoxidase (MPO Produits)
- Synonymes
- mKIAA4033 Protein, MPO Protein, LOC100335032 Protein, POX2' Protein, XPOX2' Protein, mpo Protein, mpo-A Protein, pmr-1 Protein, pmr1 Protein, pox2 Protein, xpox2 Protein, myeloperoxidase Protein, myeloperoxidase L homeolog Protein, MPO Protein, Mpo Protein, LOC100335032 Protein, mpo.L Protein
- Sujet
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Abbreviation: Myeloperoxidase,MPO
Target Synonym: Myeloperoxidase,MPO
Background: Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of the signal sequence and part of the propeptide. Human and mouse MPO share 87 % aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen-containing phagosomes.
- Poids moléculaire
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Calculated MW: 80.3 kDa
Observed MW: 85-95 kDa
- UniProt
- P05164
- Pathways
- Chromatin Binding
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