HPSE Protein (AA 1-535) (His tag)

N° du produit ABIN7505751

Détail du produit

Antigène: HPSE (Heparanase (HPSE))

Type de proteíne: Recombinant

Attributs du protein: AA 1-535

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette HPSE protéine est marqué à la His tag.

Séquence: Met1-Ile535

Attributs du produit: A DNA sequence encoding the Mouse HPA protein (Q6YGZ1) (Met1-Ile535) was expressed with a C-His.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: HPSE (Heparanase (HPSE))

Autre désignation: Heparanase (HPSE Produits)

Synonymes: im:7144134 Protein, hpa Protein, hpa1 Protein, hpr1 Protein, hpse1 Protein, hse1 Protein, HPSE Protein, HPA Protein, HPA1 Protein, HPR1 Protein, HPSE1 Protein, HSE1 Protein, Hpa Protein, Hpr1 Protein, Hep Protein, heparanase Protein, HPSE Protein, hpse Protein, Hpse Protein

Sujet:

Abbreviation: Heparanase,HPA

Target Synonym: Heparanase,EC 3.2.1.166,Endo-glucoronidase,Hpse,Hpa

Background: Heparanase (HPSE) selectively cleaves heparan sulfate (HS) at specific sites on HS proteoglycans (HSPGs). The enzyme is synthesized as an inactive 65 kDa proenzyme that is secreted via the Golgi apparatus and associates with the cell membrane through interaction with HSPGs. It is then endocytosed and transferred to lysosomes where cathepsin L activates it by removing an internal inhibitory peptide, forming a heterodimer composed of an 8 kDa and a 50 kDa subunit. Under certain stimuli, the active enzyme is transferred back to the cell surface, where it participates in extracellular matrix degradation and remodeling. HPSE facilitates cell migration associated with metastasis, wound healing and inflammation. An increase in its activity is associated with an increase in VEGF activity, which further enhances angiogenesis. HPSE also enhances shedding of syndecans and increases endothelial invasion and angiogenesis in myelomas. It acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. In addition, it increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. HPSE is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Mouse HPSE shows 76 % identity to human HPSE at amino acid sequence. The enzyme activity of recombinant mouse HPSE was assayed using recombinant syndecan 4 that was biotinylated at the non-reducing end of its HS chains in ELISA format.

Poids moléculaire:

Calculated MW: 58.74 kDa

Observed MW: 70 kDa

UniProt: Q6YGZ1

Pathways: Glycosaminoglycan Metabolic Process