E-cadherin Protein (His tag)

N° du produit ABIN7535470

Détail du produit

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette E-cadherin protéine est marqué à la His tag.

Fonction: Active Recombinant Human CDH1/E-Cadherin/CD324 Protein

Séquence: DWVIPPISCP ENEKGPFPKN LVQIKSNKDK EGKVFYSITG QGADTPPVGV FIIERETGWL KVTEPLDRER IATYTLFSHA VSSNGNAVED PMEILITVTD QNDNKPEFTQ EVFKGSVMEG ALPGTSVMEV TATDADDDVN TYNAAIAYTI LSQDPELPDK NMFTINRNTG VISVVTTGLD RESFPTYTLV VQAADLQGEG LSTTATAVIT VTDTNDNPPI FNPTTYKGQV PENEANVVIT TLKVTDADAP NTPAWEAVYT ILNDDGGQFV VTTNPVNNDG ILKTAKGLDF EAKQQYILHV AVTNVVPFEV SLTTSTATVT VDVLDVNEAP IFVPPEKRVE VSEDFGVGQE ITSYTAQEPD TFMEQKITYR IWRDTANWLE INPDTGAIST RAELDREDFE HVKNSTYTAL IIATDNGSPV ATGTGTLLLI LSDVNDNAPI PEPRTIFFCE RNPKPQVINI IDADLPPNTS PFTAELTHGA SANWTIQYND PTQESIILKP KMALEVGDYK INLKLMDNQN KDQVTTLEVS VCDCEGAAGV CRKAQPVEAG LQIPA

Specificité: Asp155-Ala709

Pureté: > 95 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 0.1-0.78 μg/mL.|2.Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), >12 % will adhere specifically after 90 minutes at 37 °C.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: CDH1/E-Cadherin/CD324 (CDH1 Produits)

Synonymes: Arc-1 Protein, CD324 Protein, CDHE Protein, ECAD Protein, LCAM Protein, UVO Protein, E-cadherin Protein, cdh1 Protein, si:dz180o5.2 Protein, uvo Protein, lcam Protein, E-Cad Protein, XBcad Protein, l-cam Protein, xcdh1 Protein, cdhc-A Protein, xb-cad Protein, XTCAD-1 Protein, c-cadherin Protein, cadherin-1 Protein, uvomorulin Protein, XB-cadherin Protein, arc-1 Protein, cd324 Protein, cdhe Protein, ecad Protein, AA960649 Protein, E-cad Protein, Ecad Protein, L-CAM Protein, Um Protein, cadherin 1 Protein, si:busm1-180o5.2 Protein, cadherin 1, type 1, E-cadherin (epithelial) Protein, cadherin 1, type 1 S homeolog Protein, CDH1 Protein, si:busm1-180o5.2 Protein, cdh1 Protein, Cdh1 Protein, cdh1.S Protein

Sujet:

Description: Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Name: Arc-1,CD324,CDHE,ECAD,LCAM,UVO,CDH1,E-Cadherin, Arc-1, CD324, CDHE, ECAD, LCAM, UVO, cadherin-1,E Cadherin

ID gène: 999

UniProt: P12830

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation