CD31 Protein (His tag)

N° du produit ABIN7520398

Détail du produit

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD31 protéine est marqué à la His tag.

Fonction: Active Recombinant Human PECAM-1/CD31 Protein

Séquence: QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KSDSGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKK

Specificité: Gln28-Lys601

Pureté: > 90 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 9.7 ng/mL-1.5 μg/mL.2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA,approximately 60-70% will adhere after 30 minutes at 37°C.|3.Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD31 Antibody at 1μg/mL (100 μL/well) can bind Human CD31 Protein with a linear range of 4-25.8 ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Détail du antigène

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM-1/CD31 (PECAM1 Produits)

Synonymes: CD31 Protein, CD31/EndoCAM Protein, GPIIA' Protein, PECA1 Protein, PECAM-1 Protein, endoCAM Protein, C85791 Protein, Cd31 Protein, Pecam Protein, platelet/endothelial cell adhesion molecule 1 Protein, platelet and endothelial cell adhesion molecule 1 Protein, pecam1 Protein, PECAM1 Protein, Pecam1 Protein

Sujet:

Description: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

Name: C85791, Cd31, PECAM-1, Pecam,PECAM1,CD31, PECA1, GPIIA', endoCAM, CD31/EndoCAM,CD31/EndoCAM,PECA1,PECAM-1,endoCAM,GPIIA

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization