The sequence of the extracellular domain of human CD64 (Gln 16-Leu 281) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Purification
Nickel and SEC
Pureté
> 95 % by SEC-HPLC
niveau d'endotoxine
<1.0 EU per mg
Biological Activity Comment
Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RI / CD64 protein can bind to anti-HER2 human IgG1 (trastuzumab) immobilized on a Protein A chip with an affinity constant (KD) of 0.1 nM.
FCGR1A
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 97 % by SDS-PAGE.
Active
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
Concentration
1 mg/mL
Buffer
PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
Agent conservateur
Without preservative
Stock
RT,4 °C,-20 °C,-80 °C
Stockage commentaire
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
Date de péremption
12 months
Antigène
FCGR1A
(Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, FcgammaRI Protein, Fcgr1 Protein, Fcgr1b Protein, AI323638 Protein, AV092959 Protein, IGGHAFC Protein, FCGR1A Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1a Protein, Fcgr1 Protein
Sujet
CD64, CD64A, FCGR1, FCGRI, FCGR1A, FCGRIA, FCR1, FCRI, IGFR1 Background: IGFRI,High affinity immunoglobulin gamma Fc receptor I, also known as FcγRI or CD64, is a type I integral membrane glycoprotein. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes, macrophages, dendritic cells and activated granulocytes. CD64 binds with high affinity to the Fc domain of IgG and it plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). CD64 is structurally composed of three extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD64 is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD64.