FCGR2B Protein (AA 46-217) (His-Avi Tag)

N° du produit ABIN7536869

Détail du produit

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Type de proteíne: Recombinant

Attributs du protein: AA 46-217

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR2B protéine est marqué à la His-Avi Tag.

Application: SDS-PAGE (SDS), Surface Plasmon Resonance (SPR), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)

Fonction: Human Fc gamma RIIb / CD32b protein

Séquence: APPKAVLKLE PQWINVLQED SVTLTCRGTH SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIVLRCHSW KDKPLVKVTF FQNGKSKKFS RSDPNFSIPQ ANHSHSGDYH CTGNIGYTLY SSKPVTITVQ APGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H

Specificité: IgG

Attributs du produit: The sequence of the extracellular domain of human CD32b (Ala 46-Pro 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Purification: Nickel and SEC

Pureté: > 95 % by SEC-HPLC

niveau d'endotoxine: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIb / CD32b protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 15 μM.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Date de péremption: 12 months

Détail du antigène

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Autre désignation: CD32b (FCGR2B Produits)

Synonymes: CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein

Sujet: CD32B, FCGR2B, FCGRIIB, FCR2B, FCRIIB, IGFR2B, IGFRIIB
Background: Low affinity immunoglobulin gamma Fc receptor IIb, also known as FcγRIIb or CD32b, is a type I integral membrane glycoprotein. CD32b is a member of the immunoglobulin superfamily and is expressed on B cells, subsets of monocytes, macrophages and granulocytes, platelets and mast cells. CD32b binds monomeric IgG with low affinity but is efficient at binding immune complexes and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation. CD32b is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail containing the immunoreceptor tyrosine-based inhibition (ITIM) motif. The product provided only contains the extracellular portion of CD32.

Poids moléculaire: 23.8 kDa

UniProt: P31994

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling