Purified recombinant Human AKR1 B10 protein Expression System: E.coli Bioactivity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 °C. Specific activity was expressed as units/mg protein. Molecular weight on SDS-PAGE will appear higher.
AKR1B10
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Indications d'application
AKR1B10 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Procédure de l'essai
Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH. 2. Add 50 µL of recombinant AKR1B10 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer . 3. Mix by inversion and Incubate at 25 °C for 2.5 minutes. 4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340 nm for 3 minutes.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
RT/-20 °C
Stockage commentaire
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Antigène
AKR1B10
(Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
AKR1B11 Protein, AKR1B12 Protein, ALDRLn Protein, ARL-1 Protein, ARL1 Protein, HIS Protein, HSI Protein, 2310005E10Rik Protein, Akr1b16 Protein, AKR Protein, AKR1B10 Protein, Akr1b10 Protein, aldo-keto reductase family 1 member B10 Protein, aldo-keto reductase family 1, member B10 (aldose reductase) Protein, aldo-keto reductase family 1 member B10-like 2 Protein, AKR1B10 Protein, Akr1b10 Protein, AKR1B10L2 Protein, LOC100585902 Protein, LOC100723118 Protein, LOC101107697 Protein
Sujet
AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography. Alternative Names: AKRB0 1, AKR1B12 protein, member B10 protein, AKRB0-1 protein, AKR1B10 protein, HIS protein, SI reductase protein, HIS protein, Aldose reductase related protein protein, AKR1B10, AKRB0-1, ARL1 protein, Aldo-keto reductase family 1 protein, Small intestine reductase., member B10 Aldose reductase like protein, ARL-1 protein, ALDRLn protein, AKRB0 1 protein, hARP protein, ARL 1 protein, AKR1B11 protein, Aldo-keto reductase family 1 protein