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GSTA1 Protein (AA 1-222)

GSTA1 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90 % pure SDS Active
N° du produit ABIN935078
  • Antigène Voir toutes GSTA1 Protéines
    GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 1-222
    Origine
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 10
    • 8
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Séquence
    MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
    Attributs du produit
    Purified recombinant Human GSTA1 protein
    Expression System: E.coli
    Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.
    Pureté
    > 90 % pure
    Top Product
    Discover our top product GSTA1 Protéine
  • Indications d'application
    GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Procédure de l'essai
    1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
      2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
      3.2 % (v/v) ethanol.
      2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
      3. Add 50 µL of recombinant GSTA1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
      4. Mix by inversion and record the increase at A340 nm for 5 minutes.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
    Agent conservateur
    Dithiothreitol (DTT)
    Précaution d'utilisation
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    RT/-20 °C
    Stockage commentaire
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Antigène
    GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))
    Autre désignation
    GSTA1 (GSTA1 Produits)
    Synonymes
    GST2 Protein, GSTA1-1 Protein, GTH1 Protein, Gst2-1 Protein, OTTMUSG00000031890 Protein, GSTA3 Protein, GST Protein, GSTA1 Protein, gst2 Protein, gth1 Protein, gsta1-1 Protein, Gsta1 Protein, Gst-2 Protein, Gst-3 Protein, Gst2 Protein, Gst3 Protein, glutathione S-transferase alpha 1 Protein, glutathione S-transferase, alpha 1 (Ya) Protein, glutathione S-transferase alpha 3 Protein, glutathione S-transferase alpha 5 Protein, glutathione S-transferase alpha 1 L homeolog Protein, glutathione S-transferase alpha 2 Protein, glutathione S-transferase mu 2 (muscle) Protein, Glutathione S-transferase 2 Protein, glutathione S-transferase A Protein, glutathione S-transferase Gst2 Protein, glutathione S-transferase 2 Protein, GSTA1 Protein, Gsta1 Protein, GSTA3 Protein, GSTA5 Protein, gsta1.L Protein, gsta1 Protein, Gsta2 Protein, GSTM2 Protein, gst-2 Protein, LOC100713029 Protein, gst2 Protein, LOC101897277 Protein
    Sujet
    GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: GSTA-1 protein, GSTA 1 protein, HA subunit 1., GTH1 protein, Glutathione S-transferase alpha 1 Glutathione S transferase A1 protein, GSTA1 1 protein, GSTA1, GSTA-1, GST epsilon protein, GSTA1-1 protein, GST2 protein, GSTA 1, GSTA1 protein
    Poids moléculaire
    25.6 kDa (222 AA)
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