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FKBP3 Protein (AA 1-224)

FKBP3 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90 % pure SDS Active
N° du produit ABIN935093
  • Antigène Voir toutes FKBP3 Protéines
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 1-224
    Origine
    • 8
    • 2
    • 2
    • 1
    • 1
    Humain
    Source
    • 9
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Séquence
    MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID
    Attributs du produit
    Purified recombinant Human FKBP3 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 120 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
    Pureté
    > 90 % pure
    Top Product
    Discover our top product FKBP3 Protéine
  • Indications d'application
    FKBP3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Procédure de l'essai
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant FKBP3 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
    Agent conservateur
    Dithiothreitol (DTT)
    Précaution d'utilisation
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    RT/-20 °C
    Stockage commentaire
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Antigène
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Autre désignation
    FKBP3 (FKBP3 Produits)
    Synonymes
    FKBP3 Protein, 25kDa Protein, FKBP-3 Protein, FKBP25 Protein, FK506 Protein, fb75c04 Protein, si:dz261o22.2 Protein, wu:fb75c04 Protein, zgc:110728 Protein, FKBP-25 Protein, PPIase Protein, FK506 binding protein 3 Protein, FK506 binding protein 3 L homeolog Protein, peptidylprolyl isomerase Protein, FKBP3 Protein, Fkbp3 Protein, fkbp3 Protein, fkbp3.L Protein, CAALFM_C103790CA Protein
    Sujet
    FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: PPIase protein, FK506 binding protein 3 FKBP 25 protein, FKBP-3 protein, FKBP3, PPIase FKBP3 protein, FKBP-3, Rotamase protein, Rotamase., FKBP25 protein, FKBP 3, FKBP 3 protein, FKBP 3 protein
    Poids moléculaire
    25.1 kDa (224 AA)
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