Purified recombinant Human FKBP3 protein Expression System: E.coli Bioactivity: Specific activity is > 120 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
FKBP3
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Indications d'application
FKBP3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Procédure de l'essai
Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA). 2. Add recombinant FKBP3 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer. 3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
RT/-20 °C
Stockage commentaire
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques. Alternative Names: PPIase protein, FK506 binding protein 3 FKBP 25 protein, FKBP-3 protein, FKBP3, PPIase FKBP3 protein, FKBP-3, Rotamase protein, Rotamase., FKBP25 protein, FKBP 3, FKBP 3 protein, FKBP 3 protein