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Protein A Protein (HRP)

Origine: Staphylococcus aureus Recombinant ELISA, WB
N° du produit ABIN964521
  • Antigène Voir toutes Protein A Protéines
    Protein A
    Type de proteíne
    Recombinant
    Origine
    Staphylococcus aureus
    Hôte
    Veuillez nous consulter SVP
    Purification/Conjugué
    Cette Protein A protéine est marqué à la HRP.
    Application
    ELISA, Western Blotting (WB)
    Specificité
    Protein A Peroxidase Conjugated was prepared from chromatographically pure Protein A. Protein A Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
    Top Product
    Discover our top product Protein A Protéine
  • Indications d'application
    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).
    Commentaires

    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    2.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Agent conservateur
    Gentamicin sulfate
    Conseil sur la manipulation
    Do NOT add Sodium Azide!
    Stock
    4 °C
    Date de péremption
    12 months
  • Antigène
    Protein A
    Abstract
    Protein A Produits
    Synonymes
    alpha protein Protein, repB Protein
    Sujet
    Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
    Synonyms: HRP, peroxidase, Horseradish peroxidase protein A
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