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Protein A Protein (Alkaline Phosphatase (AP))

Origine: Staphylococcus aureus Recombinant ELISA
N° du produit ABIN964522
  • Antigène Voir toutes Protein A Protéines
    Protein A
    Type de proteíne
    Recombinant
    Origine
    Staphylococcus aureus
    Hôte
    Veuillez nous consulter SVP
    Purification/Conjugué
    Cette Protein A protéine est marqué à la Alkaline Phosphatase (AP).
    Application
    ELISA
    Specificité
    Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
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    Discover our top product Protein A Protéine
  • Indications d'application
    Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
    Commentaires

    Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Date de péremption
    12 months
  • Antigène
    Protein A
    Abstract
    Protein A Produits
    Synonymes
    alpha protein Protein, repB Protein
    Sujet
    Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
    Synonyms: ProA, ALP, Alk Phos
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