EGF Protein (AA 971-1023)

N° du produit ABIN987814

Détail du produit

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 971-1023

Origine: Humain

Source: Escherichia coli (E. coli)

Attributs du produit: ED50 <0.6 ng/mL, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of >1.7 x 10^6 units/mg.
AA 971-1023, expressed with an N-terminal Met

Pureté: > 95 % by SDS-PAGE analysis.

niveau d'endotoxine: < 0.1 ng/μg (1 EU/μg) determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80 °C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4 °C or up to 6 months at -20 °C.

Date de péremption: 12 months

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: Epidermal Growth Factor (EGF Produits)

Sujet: Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-a and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Urogastrone, URG

Poids moléculaire: 6.2kDa, observed by reducing SDS-PAGE.

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation