Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human activated lymphocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.
Pureté
> 97 % by SDS-PAGE and HPLC analyses.
niveau d'endotoxine
Level Less than 1EU/µg of rMuMDC/CCL22 as determined by LAL method
CCL22
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 96 % by SDS-PAGE and HPLC analyses.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoattracts monocytes, dendritic cells and NK cells and exerts HIV suppressive activity. Synonym: MDC/CCL22, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Poids moléculaire
7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.