Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.
Pureté
> 97 % by SDS-PAGE and HPLC analyses.
niveau d'endotoxine
Level Less than 1EU/µg of rMuMEC/CCL28 as determined by LAL method
CCL28
Origine: Humain
Hôte: Escherichia coli (E. coli)
> 97 % by SDS-PAGE and HPLC analyses.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag1 mouse kidney cDNA library. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK. Synonym: MEC/CCL28, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4,150mM NaCl.
Poids moléculaire
12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.