AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KA
Attributs du produit
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human neutrophils is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.
Pureté
> 97 % by SDS-PAGE and HPLC analyses.
niveau d'endotoxine
Level Less than 1EU/µg of rMuMIP-2/CXCL2 as determined by LAL method
CXCL2
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Macrophage Inflammatory Protein 2 (MIP-2) was originally identified as a heparin binding protein secreted from a mouse macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (CXC) subfamily of chemokines.Similarly to other alpha chemokines, mouse MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the mouse interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases. Synonym: MIP-2/CXCL2, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Poids moléculaire
7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.