Lapin anti-Poulet IgG (Heavy & Light Chain) Anticorps - Preadsorbed

N° du produit ABIN101033

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG (H&L)

Réactivité croisée: Poulet

Attributs du produit: Anti-Chicken IgG antibody generated in rabbit detects specifically chicken IgG. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:200,000 - 1:400,000

Western Blot Dilution: 1:10,000 - 1:30,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Rabbit anti-Chicken IgG Antibody, Chicken Secondary Antibody

Background: Anti-Chicken IgG Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.