Lapin anti-Poulet IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- F(ab')2 Region
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Reactivité
- Poulet
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- IgG F(ab')2
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Stérilité
- Sterile filtered
- Immunogène
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Immunogen: Chicken IgG F(ab')2 fragment
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Chicken IgG F(ab')2 antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:3,500
Western Blot Dilution: 1:500 - 1:2,500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: rabbit anti-Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugation, rabbit anti-Chicken IgG Fab2 Fragment Alk Phos Conjugated Antibody
Background: Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
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