Lapin anti-Chévre IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed
-
- Antigène Tous les produits IgG
- IgG
-
Épitope
- F(ab')2 Region
-
Reactivité
- Chévre
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Alkaline Phosphatase (AP)
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- IgG F(ab')2
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Stérilité
- Sterile filtered
- Immunogène
-
Immunogen: Goat IgG F(ab')2 fragment
- Isotype
- IgG
-
-
- Indications d'application
-
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: This product has been assayed against 1.0 μg of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 is suggested for this product.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
-
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Stock
- 4 °C
- Date de péremption
- 12 months
-
- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
-
Synonyms: rabbit anti-goat IgG F(ab')2 ALP conjugated, rabbit anti-goat IgG F(ab')2 alkaline phosphatase conjugated, rabbit anti-goat IgG Fab2 ALP conjugated, rabbit anti-goat IgG Fab2 alkaline phosphatase conjugated
Background: Anti-Goat IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.
-