Chèvre anti-Cobaye IgG (F(ab')2 Region) Anticorps (FITC) - Preadsorbed

N° du produit ABIN101310

Détail du produit

Antigène: IgG

Épitope: F(ab')2 Region

Reactivité: Cobaye

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificité: IgG F(ab')2

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Guinea Pig IgG F(ab')2 fragment

Isotype: IgG

Labeling Ratio: 2.8

Information d'application

Indications d'application:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: goat Anti-Guinea Pig IgG F(ab')2 Antibody Fluorescein Conjugated, goat Anti-Guinea Pig IgG Fab2 Fragment Antibody FITC Conjugated

Background: Anti-Guinea Pig IgG F(ab')2 Fluorescein Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.