Lapin anti-Humain IgG (F(ab')2 Region) Anticorps - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- F(ab')2 Region
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- IgG F(ab')2
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: immunoaffinity chromatography using Human antigens coupled to agarose beads
- Stérilité
- Sterile filtered
- Immunogène
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Immunogen: Human IgG F(ab')2 fragment
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Human IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:25,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 6.03 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: Rabbit Anti Human IgG Fab2 Antibody, Rabbit Anti-Human IgG F(ab')2 Antibody, Rabbit Anti Human IgG Fab2 Fragment Antibody
Background: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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