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Lapin anti-Humain IgG (F(ab')2 Region) Anticorps - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
N° du produit ABIN101618
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2352
    • 512
    • 418
    • 206
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivité
    • 725
    • 699
    • 601
    • 472
    • 400
    • 239
    • 208
    • 176
    • 146
    • 137
    • 112
    • 107
    • 104
    • 101
    • 83
    • 59
    • 48
    • 34
    • 28
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    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 1776
    • 1551
    • 567
    • 250
    • 112
    • 110
    • 33
    • 31
    • 21
    • 21
    • 15
    • 11
    • 11
    • 1
    Lapin
    Clonalité
    • 3403
    • 188
    • 1
    Polyclonal
    Application
    • 1953
    • 1836
    • 1803
    • 1509
    • 917
    • 894
    • 649
    • 478
    • 380
    • 236
    • 236
    • 233
    • 107
    • 102
    • 86
    • 68
    • 54
    • 53
    • 31
    • 28
    • 25
    • 20
    • 18
    • 15
    • 11
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgG F(ab')2
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Human antigens coupled to agarose beads
    Stérilité
    Sterile filtered
    Immunogène

    Immunogen: Human IgG F(ab')2 fragment

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:25,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    6.03 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit Anti Human IgG Fab2 Antibody, Rabbit Anti-Human IgG F(ab')2 Antibody, Rabbit Anti Human IgG Fab2 Fragment Antibody

    Background: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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